Fig. 1

Generation of DIO mice by high-fat diet feeding. a Changes in body weights in C57BL/6 mice fed with normal CHOW (CHOW, n = 14) or high fat diet (DIO, n = 37). Inset: pictures of CHOW (a) and DIO (b) mice. b Average food intake per mouse per 24-hour period (n = 14 in CHOW and n = 22 in DIO). c Intraperitoneal glucose tolerance test (GTT) of DIO mice and CHOW controls. d Area under the curve of GTT. e Insulin tolerance test (ITT) of DIO mice and CHOW mice. f Reverse area under the baseline above the curve, n = 5–10 in each group. *P < 0.05; **P < 0.01, analysis-of-variance test. CHOW mice fed standard diet, DIO diet-induced obese mice treated with vehicle