Fig. 5

Immunofluoroscent staining of pancreases from CHOW, DIO, and DIO + ASC mice. a Representative staining of pancreatic islets from CHOW, DIO, and DIO + ASC mice. Red represents insulin + cells. Blue stains for nuclei. Bar = 100 μm. b Statistics of islets/β-cell area in mice from each group. At least three mice were included in each group. c Immunofluorescent staining for GFP⁺ cells under fluorescent microscope. Arrows pointed to GFP⁺ cells. Scale bar = 100 μm. At least three mice were analyzed in each group; *P < 0.05, analysis-of-variance test. Relative mRNA expression of NOD2 (d), F4/80 (e), TNF-α (f), and IL-6 (g) in pancreases of CHOW, DIO, and DIO + ASC (ASC) mice as measured by reverse transcription-polymerase chain reaction analysis. *P < 0.05. ASC adipose-derived stem cell, CHOW mice fed standard diet, DIO diet-induced obese mice treated with vehicle, NOD2 nucleotide-binding oligomerization domain containing 2, TNF-α tumor necrosis factor-alpha