Skip to main content

Advertisement

Fig. 5 | Stem Cell Research & Therapy

Fig. 5

From: Adult bone marrow mesenchymal and neural crest stem cells are chemoattractive and accelerate motor recovery in a mouse model of spinal cord injury

Fig. 5

Effects of MSC- and NCSC-secretome on RAW264.7 macrophages migration and metabolic activity in vitro. We evaluated the chemoattractive properties of MSC and NCSC secretome by placing RAW264.7 macrophages on the top of a 5 μm-filter, with MSC-CM, LPSMSC-CM, NCSC-CM or LPSNCSC-CM in the bottom chamber (a). After 20 h, migration rate was assessed by the evaluation of the filter area occupied by macrophages. Passive migration was observed in response to serum-free DMEM (b, h), but drastically increased in response to LPS (c, h). MSC-CM slightly increased macrophage migration (d, h), but LPSMSC-CM was more efficient (e, h). NCSC-CM did not significantly induce macrophage migration (f, h), while LPSNCSC-CM also increased it (g, h). MTS metabolic assay also showed that LPSMSC-CM and LPSNCSC-CM increased the metabolic activity of RAW264.7 cells i (n = 3, one-way ANOVA and HSD post-test, **p < 0.01, ***p < 0.001). Scale bar = 50 μm. MSC mesenchymal stem cell, NCSC neural crest stem cell, CM conditioned medium, LPS lipopolysaccharide, DMEM Dulbecco’s modified Eagle’s medium, ANOVA analysis of variance, HSD honestly significant difference

Back to article page