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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: Endothelial properties of third-trimester amniotic fluid stem cells cultured in hypoxia

Fig. 2

Selection, expansion of amniotic fluid stem cells and characterization of obtained cultures. a, b Cells contained in amniotic fluid of both trimesters were seeded over coverslips and then selected for CD117 antigen for subsequent expansion in culture, and two different oxygen concentrations were used for the experiments (20 % and 5 %). The obtained cells were then characterized for surface markers; here are representative data for the cells obtained from the second (c) and the third (d) trimester with their percentage of positive cells for each specific marker: red lines and numbers = normoxic condition (20 % O2); blue lines and numbers = hypoxic condition (5 % O2). The presence of the initial marker for selection (CD117) was monitored during the different passages in culture for second-trimester (e) and third-trimester (f) cells

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