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Table 1 List of antibodies

From: Endothelial properties of third-trimester amniotic fluid stem cells cultured in hypoxia

Antibody Conjugation Dilution/procedure Manufacturer
CD9 FITC   Biolegend
CD29 FITC   Biolegend
CD31 FITC   Biolegend
CD34 FITC   Miltenyi Biotec
CD44 FITC   eBioscience
CD45 FITC   BD Pharmingen
CD56 PE   Beckman Coulter
CD73 PE   Biolegend
CD90 FITC   Biolegend
CD105 PE   Beckman Coulter
CD117 APC   Miltenyi Biotec
CD146 FITC   BioCytex
CD166 PE   Beckman Coulter
CD184 PE   Beckman Coulter
CD271 PE   Miltenyi Biotec
HLA-ABC FITC   Beckman Coulter
HLA-DR PE   Beckman Coulter
vWF   1:100; 1 hour; 37 °C Dako
Anti-human mitochondria   1:80; 1 hour; 37 °C Abcam
Anti-mouse CD31   1:80; overnight; 37 °C Millipore
Anti-human CD31   1:80; overnight; 37 °C Chemicon Europe
SSEA4   1:100; 1 hour; 37 °C Abcam
Chicken anti-rabbit Alexafluor 488 1:150; 1 hour; 37 °C Life Technologies
Goat anti-mouse Alexafluor 594 1:150; 1 hour; 37 °C Life Technologies
Hamster anti-mouse Alexafluor 594 1:150; 1 hour; 37 °C Life Technologies
  1. FITC fluorescein isothiocyanate, PE phycoerythrin, APC allophycocyanin, vWF von Willebrand factor, SSEA4 stage-specific embryonic antigen 4