Endothelial properties of third-trimester amniotic fluid stem cells cultured in hypoxia

Table 1 List of antibodies

Antibody	Conjugation	Dilution/procedure	Manufacturer
CD9	FITC		Biolegend
CD29	FITC		Biolegend
CD31	FITC		Biolegend
CD34	FITC		Miltenyi Biotec
CD44	FITC		eBioscience
CD45	FITC		BD Pharmingen
CD56	PE		Beckman Coulter
CD73	PE		Biolegend
CD90	FITC		Biolegend
CD105	PE		Beckman Coulter
CD117	APC		Miltenyi Biotec
CD146	FITC		BioCytex
CD166	PE		Beckman Coulter
CD184	PE		Beckman Coulter
CD271	PE		Miltenyi Biotec
HLA-ABC	FITC		Beckman Coulter
HLA-DR	PE		Beckman Coulter
vWF		1:100; 1 hour; 37 °C	Dako
Anti-human mitochondria		1:80; 1 hour; 37 °C	Abcam
Anti-mouse CD31		1:80; overnight; 37 °C	Millipore
Anti-human CD31		1:80; overnight; 37 °C	Chemicon Europe
SSEA4		1:100; 1 hour; 37 °C	Abcam
Chicken anti-rabbit	Alexafluor 488	1:150; 1 hour; 37 °C	Life Technologies
Goat anti-mouse	Alexafluor 594	1:150; 1 hour; 37 °C	Life Technologies
Hamster anti-mouse	Alexafluor 594	1:150; 1 hour; 37 °C	Life Technologies

FITC fluorescein isothiocyanate, PE phycoerythrin, APC allophycocyanin, vWF von Willebrand factor, SSEA4 stage-specific embryonic antigen 4

ISSN: 1757-6512