Table 2 Primer sequences used for mRNA quantification
From: Endothelial properties of third-trimester amniotic fluid stem cells cultured in hypoxia
Gene | NM_ | Forward primer | Reverse primer | Amplicon, base pairs |
|---|---|---|---|---|
ETV2 | 014209.2 | AGTCGGACCGTGCCAGTTTG | GAGCCACCTCTTTGGGGTCG | 168 |
FLI1 | 002017.4 | *GAGGAGCTTGGGGCAATAAC | *AGAGCAGCTCCAGGAGGAAT | 195 |
VEGF-A | all isoforms | TCACCATGCAGATTATGCGGA | TGTTGTGCTGTAGGAAGCTCA | 75 |
B2M | 004048.2 | CAACTTCAATGTCGGATGGATG | GCTGTGCTCGCGCTACTCT | 161 |