Skip to main content
Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: Cellular magnetic resonance imaging contrast generated by the ferritin heavy chain genetic reporter under the control of a Tet-On switch

Fig. 3

Immunostaining of doxycycline-regulated Flag expression in C3H10T1/2-FTH1 cells. The cells were grown in different concentrations of Dox for 72 h. Immunostaining using a Flag-specific antibody demonstrated that with increasing Dox concentration, the expression of Flag in C3H10T1/2-FTH1 cells increased and peaked upon induction with 0.2 μg/ml Dox for 72 h and then decreased (red); this result is consistent with the western blot results. The nucleus was visualized via DAPI staining (blue). Bar = 50 μm. FTH1 ferritin heavy chain, Dox doxycycline, DAPI 4,6-diamidino-2-phenylindole

Back to article page