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Fig 3 | Stem Cell Research & Therapy

Fig 3

From: Generation of clinical-grade human induced pluripotent stem cells in Xeno-free conditions

Fig 3

Clinical-grade hiPSCs can randomly differentiate into all three germ layers and directly differentiate into functional neural cells and cardiomyocytes in Xeno-free media. a Morphology of EBs at day 8 derived from one clinical-grade hiPSC line. Scale bar, 200 μm. b Gene expression profile for germ-layer marker genes of the day 8 EBs, ectoderm (Gad1 and Pax6), mesoderm (Enolase and Osteonectin), endoderm (Nicastrin and Alpha-fetoprotein), and pluripotency (Oct4, Sox2 and Nanog). c Hematoxylin and eosin staining of teratoma derived from one clinical-grade hiPSC line. Scale bar, 100 μm. The teratomas contain tissues of all three germ layers. d Immunofluorescence results of neural cells differentiated from one clinical-grade hiPSC line. The neural cells express TH1 and TUJ1 at protein level. Scale bar, 100 μm. e Immunofluorescence results of cardiomyocytes differentiated from one clinical-grade hiPSC line. The cardiomyocytes express NKX2.5 and CTNT at protein level. Scale bar, 100 μm. EB embryoid body, GMP good manufacturing practice, iPSC induced pluripotent stem cell

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