Fig. 1

Properties of hASC-HLCs cultured on collagen type I-coated glass slides and on 2D-AHAM. SEM shows the morphology of hASC-HLCs cultured on collagen type I-coated glass slides (a) and on 2D-AHAM (b) for 72 hours in vitro. Immunofluorescence staining of human MRP2 of hASC-HLCs cultured on collagen type I-coated glass slides (c) and on 2D-AHAM (d). Arrow shows the location of the BC. e Real-time RT-PCR was used to analyze the expression of hepatocyte function-specific genes of hASC-HLCs plated on different substrates. The freshly differentiated hASC-HLCs (indicate that differentiated cells do not trypsinize and reseed after the end of the differentiated program of 21 days) and human hepatocytes were used as controls. The relative expression of each gene was normalized to 18S rRNA. *Statistically significant compared with the hASC-HLCs cultured on collagen type I (p <0.05). f Levels of ALB secreted by the hASC-HLCs cultured on different substrates as analyzed by ELISA. CYP cytochrome, 2D-AHAM cryopreserved and dried acellular human amniotic membrane, hASC human adipose stem cell, HLC hepatocyte-like cell