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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: Transplantation of human adipose stem cell-derived hepatocyte-like cells with restricted localization to liver using acellular amniotic membrane

Fig. 2

Properties of hASC-HLCs cultured on 3D-AHAM in vitro . Macroscopic appearance of the hASC-HLC–3D-AHAM cultured on day 1 (a) and day 3 (b) in vitro. Macroscopic appearance of the hASC-HLC–2D-AHAM cultured on day 3 in vitro (b) (insert). H&E staining showed that the hASC-HLCs attached to the AHAM intensely and formed a 3D nest-like structure (c, d) on day 3. Scale bars: 100 μm (c), 50 μm (d). e Immunofluorescence staining of ALB of hASC-HLC–3D-AHAM on day 3. The negative control using isotype IgG for immunofluorescence (e) (insert). Scale bar: 50 μm. f TEM analysis of the ultrastructure of hASC-HLC–3D-AHAM on day 3. Scale bar: 1 μm. AHAM acellular human amniotic membrane, BC bile canaliculus, Mt mitochondria, N nucleus, rER rough endoplasmic reticulum, TJ tight junction

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Stem Cell Research & Therapy

ISSN: 1757-6512