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Fig. 7 | Stem Cell Research & Therapy

Fig. 7

From: Transplantation of human adipose stem cell-derived hepatocyte-like cells with restricted localization to liver using acellular amniotic membrane

Fig. 7

Long-term properties of the hASC-HLC–3D-AHAM graft in the injured liver following transplantation. a H&E staining analysis of the graft at 8 weeks post implantation. Black arrows show the location of the vessel-like structure. White arrows show the hASC-HLCs with the cubic morphology. Immunofluorescence staining of human nuclei (b), ALB (c), HNF4α (d), CYP2B6 (e), and mouse CD31 (f) in the hASC-HLC–3D-AHAM graft in the CCl4-injured liver at 8 weeks post implantation. The control of immunofluorescence using anti-human nuclei was analyzed in tissues from nontransplanted mice (d) (insert). Black arrows show the location of the vessel-like structure. Dotted lines show the location of the grafts. Scale bar: 75 μm (b), (c), 100 μm (a), (d). AHAM acellular human amniotic membrane

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