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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: A puromycin selectable cell line for the enrichment of mouse embryonic stem cell-derived V3 interneurons

Fig. 3

Puromycin selection increases the percentage of cells expressing Sim1 in Sim1-Puro cultures. ac Sim1 in situ hybridization (dark cell bodies) and nuclear staining (blue) on unselected cells. df Sim1 in situ hybridization and nuclear staining on selected cells. a & d Sim1 in situ hybridization. b & e Nuclear marker Hoechst (blue). c & f Overlaid images. g Selected cultures enriches for percentage of Sim1+ cells. * Denotes P <0.05 compared to unselected group

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