Fig. 4

Sim1-Puro cultures exhibit glutamatergic and neuronal markers through the first week post-selection. a–d Staining 3 days post-selection. e–h Staining 7 days post-selection. Immunocytochemistry marker labeled on far left. a & e β-III tubulin (red in combined); b & f VGluT2 (green in combined); c & g Combined image; d & h Zoomed in combined image. Scale bar = 100 μm