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Fig. 5 | Stem Cell Research & Therapy

Fig. 5

From: A puromycin selectable cell line for the enrichment of mouse embryonic stem cell-derived V3 interneurons

Fig. 5

Sim1-Puro cultures exhibit synaptic marker protein and mRNA expression after 2 weeks. af Two-week selected cultures stained with dendritic marker MAP2 and synaptic markers, SV2 and Bassoon. Synaptic markers (b and e, red) exhibit puncta that overlap with MAP2 (c and f, white arrows) suggesting synapse formation. Scale bar = 50 μm. g Two-week selected cultures express increased synaptic marker (Bassoon, PSD95, and SV2) and VGluT2 (glutamatergic neuron) mRNA levels compared to unselected cultures (normalization control – Sim1-Puro ESCs). * Denotes P <0.05 compared to the unselected group

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