Fig. 7

Excitatory and inhibitory agonists activate appropriate whole-cell currents in Sim1-Puro selected cultures. a Whole-cell currents evoked by kainate (K), γ-aminobutyric acid (GABA), glycine (Gly), and N-methyl-D-aspartate (NMDA, N; with 1 μM added glycine). Holding potential, −80 mV. b Agonist-evoked currents recorded during voltage ramps from −100 to +50 mV at 1.2 mV/msec. GABA and Gly evoked a current that reversed polarity at −48.6 ± 2.0 mV (n = 21) and −47.7 ± 2.2 mV (n = 10), respectively, consistent with activation of channels selective for chloride. K and NMDA evoked currents reversed at −1.4 ± 5.4 mV (n = 24) and −5.3 ± 7.2 mV (n = 4), respectively, consistent with activation of cation selective channels. c Currents increased with time in culture after puromycin selection (n = 30 to 45 cells per bar). *Denotes P <0.01 (Mann–Whitney rank sum test)