Fig. 8From: A puromycin selectable cell line for the enrichment of mouse embryonic stem cell-derived V3 interneuronsSim1-Puro-selected cultures present synaptic currents consistent with glutamatergic neurons. a Whole-cell current recorded at −80 mV during exposure to Tyrode’s solution containing elevated potassium (10 mM; KCl alone) as indicated by the open box, or 10 mM potassium Tyrode’s that also included 30 μM NBQX and 50 μM APV to block AMPA/kainate and NMDA receptors, respectively, as indicated by the filled box. Short segments during each exposure are shown on a 10-fold expanded time scale. b Plots of mean ± sem amplitude (Amp, pA), frequency (freq, Hz), and width at half amplitude (half-width, msec) of synaptic events evoked during exposure to elevated KCl alone (open bars, four cells) or with 200 μM of the GABA antagonist bicuculline methiodide (grey bars, three cells)Back to article page