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Table 1 Primers for qRT-PCR genes

From: Nanochelating based nanocomplex, GFc7, improves quality and quantity of human mesenchymal stem cells during in vitro expansion

  Genes Primer sequences
1 ALP FW, 5′- GCA CCT GCC TTA CTA ACT C −3′;
RW, 5′- AGA CAC CCA TCC CAT CTC −3′;
2 COL1 FW, 5′- TGG AGC AAG AGG CGA GAG −3′;
RW, 5′- CAC CAG CAT CAC CCT TAG C −3′;
3 CXCR4 FW, 5-′ CGC CAC CAA CAG TCA GAG −3′ ;
RW, 5′- AAC ACA ACC ACC CAC AAG TC −3′;
4 GAPDH FW, 5′- GACAAGCTTCCCGTTCTCAG-3′;
RW, 5′- GAGTCAACGGATTTGGTCGT-3′;
5 H-P53 FW, 5′- GGA GTA TTT GGA TGA CAG AAA C −3′;
RW, 5′- GAT TAC CAC TGG AGT CTT C −3′;
6 LERP FW, 5′- CAA TCT GAA TGA AAC CAA ACC TC −3′;
RW, GGC TGC TCC TAT GAT ACC TC −3′;
7 REX1 FW, 5′- CGG GAC GAG GAG TGT TAT TAC −3′;
RW, 5′- CGT GTT GCT TTG CGA CTT G −3′;
8 RUNX2 FW, 5′- GCC TTC AAG GTG GTA GCC C −3′;
RW, 5′- CGT TAC CCG CCA TGA CAG TA −3′
9 Lipoprotein lipase FW, 5′- CCC TAC AAA GTC TTC CAT TAC −3′;
RW, 5′- AGT TCT CCA ATA TCT ACC TCT G −3′;
10 NRF2 FW, 5′- GCG ACG GAA AGA GTA TGA G −3′;
RW, 5′- GGG CAA CCT GGG AGT AG −3′;
11 NANOG FW, 5′- GCT AAG GAC AAC ATT GAT AGA AG −3′;
RW, 5′- CTT CAT CAC CAA TTC GTA CTT G −3′;
12 Osteonectin FW: 5′: CTCGCTTCGGCAGCACACATATAC-3′,
RW: 5′- ACGCTTCACGAATTTGCGTGTC-3′.
13 Osteocalcin FW, 5-′ GCA AAG GTG CAG CCT TTG TG −3′ ;
RW, 5′- GGC TCC CAG CCA TTG ATA CAG −3′;
14 OCT-4 FW, 5′- CGC CGT ATG AGT TCT GTG −3′;
RW, 5′- GGT GAT CCT CTT CTG CTT C −3′;
15 SOX2 FW; 5′- GGA CTG AGA GAA AGA AGA GGA G −3′
RW, 5′- GAA AAT CAG GCG AAG AAT AAT-3′;
16 SOD1 FW, 5′- CGA GCA GAA GGA AAG TAA TG −3′;
RW, 5′- TGG ATA GAG GAT TAA AGT GAG G −3′;