Fig. 3

OBC matrix slows MSC proliferation in EM and OM. MSCs were plated on plastic or DOC-treated or water-treated OBC matrix and maintained in OM or EM. Their proliferation was examined on culture days 6 and 12. a In OM, MSC proliferation was reduced by 2-fold on DOC-treated OBC matrix and 1.2-fold on water-treated matrix. b MSC proliferation on plastic was 1.3-fold lower in OM than in EM, but this reduced proliferation is absent when comparing cells in EM and OM on water-treated OBC matrix. Fold changes are compared to the pre-culture value and expressed as mean ± SEM. Data are from a single experiment, representative of three experiments. *, p < 0.5, **, p < 0.01, ***, p < 0.001. OBC osteoblastic cells, MSC mesenchymal stem cells, EM expansion medium, OM osteogenic medium, SEM standard error of the mean