Fig. 5

OBC matrix preserves the naïve, fibroblastic morphology of MSCs. Fluorescence microscopy of Calcein AM-labeled cells shows that, even at day 12, MSCs cultured in OM and seeded on OBC matrices (c, DOC- and d, water-treated) retain their fibroblastic morphology, like MSCs cultured in EM on plastic (a), instead of becoming cuboidal, like cells cultured in OM on plastic (b). a, b Viewed at day 6. OBC osteoblastic cells, MSC mesenchymal stem cells, OM osteogenic medium, EM expansion medium