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Table 1 “Pick List” of statistically significant protein spots for identification

From: Nascent osteoblast matrix inhibits osteogenesis of human mesenchymal stem cells in vitro

Spot No. Avg. ratio p-value (t-test) Spot Vol. pI Mr Match quality
201 5.32 0.019 N/A 5.65 133,581 1.80
203 5.69 0.015 1,809,098 5.72 133,294 2.38
208 6.21 0.015 1,387,720 5.81 132,723 1.78
210 7.48 0.015 2,038,969 5.90 131,588 0.74
214 7.7 0.015 2,700,438 6.00 130,462 0.52
216 7.72 0.015 2,774,451 6.09 129,903 0.47
224 5.93 0.015 11,334,140 4.82 127,965 2.77
242 6.59 0.019 1,651,536 5.24 121,536 3.41
247 4.71 0.015 2,109,428 5.39 120,497 5.05
449 7.28 0.019 1,501,211 4.76 82,040 1.30
486 −1.6 0.038 1,347,174 4.59 75,936 2.60
562 −3.64 0.026 1,435,516 6.56 66,327 2.55
565 −3.31 0.030 2,230,394 6.85 66,184 1.05
566 −1.73 0.043 258,223 7.15 66,184 2.82
607 −2.65 0.015 1,380,551 5.02 61,260 3.09
637 −2.67 0.036 4,584,544 4.16 57,933 0.77
649 −4.96 0.015 746,894 6.09 57,561 12.13
658 −1.52 0.031 773,745 6.43 56,824 3.03
689 −1.73 0.049 3,281,833 4.82 54,319 0.67
717 −2.11 0.041 5,864,175 4.42 51,923 0.70
758 −2.09 0.041 750,800 7.99 48,788 3.34
826 −2.69 0.034 2,653,198 5.71 42,890 2.37
894 −1.93 0.015 1,912,332 5.17 38,606 5.09
895 −1.83 0.041 694,589 4.97 38,275 2.33
896 −3.09 0.015 5,361,677 5.35 38,440 2.96
1053 −2.63 0.036 2,984,453 4.99 27,203 1.65
1093 −7.66 0.015 6,128,338 7.30 24,017 2.46
1095 −4.59 0.023 1,765,854 6.92 23,914 1.91
1096 −7.55 0.015 13,937,178 7.60 23,812 0.52
  1. DeCyder-generated list of proteins whose abundances vary by at least ±1.5-fold (p ≤ 0.05) between DOC- and water-treated OBC matrices. Columns show (left to right): numbers assigned to each spot on the gel; average ratios between spot volumes (DOC/water); p-values corresponding to those ratios; maximum spot volumes (pixels); approximate pIs and Mr (Daltons) based on position within the gel; and match quality, a measure of consistency between a spot’s replicates in different gels (lower is better). Spots above the double line are more abundant in DOC matrix, below, in water matrix
  2. OBC osteoblastic cell