Nascent osteoblast matrix inhibits osteogenesis of human mesenchymal stem cells in vitro

Table 1 “Pick List” of statistically significant protein spots for identification

Spot No.	Avg. ratio	p-value (t-test)	Spot Vol.	pI	M_r	Match quality
201	5.32	0.019	N/A	5.65	133,581	1.80
203	5.69	0.015	1,809,098	5.72	133,294	2.38
208	6.21	0.015	1,387,720	5.81	132,723	1.78
210	7.48	0.015	2,038,969	5.90	131,588	0.74
214	7.7	0.015	2,700,438	6.00	130,462	0.52
216	7.72	0.015	2,774,451	6.09	129,903	0.47
224	5.93	0.015	11,334,140	4.82	127,965	2.77
242	6.59	0.019	1,651,536	5.24	121,536	3.41
247	4.71	0.015	2,109,428	5.39	120,497	5.05
449	7.28	0.019	1,501,211	4.76	82,040	1.30
486	−1.6	0.038	1,347,174	4.59	75,936	2.60
562	−3.64	0.026	1,435,516	6.56	66,327	2.55
565	−3.31	0.030	2,230,394	6.85	66,184	1.05
566	−1.73	0.043	258,223	7.15	66,184	2.82
607	−2.65	0.015	1,380,551	5.02	61,260	3.09
637	−2.67	0.036	4,584,544	4.16	57,933	0.77
649	−4.96	0.015	746,894	6.09	57,561	12.13
658	−1.52	0.031	773,745	6.43	56,824	3.03
689	−1.73	0.049	3,281,833	4.82	54,319	0.67
717	−2.11	0.041	5,864,175	4.42	51,923	0.70
758	−2.09	0.041	750,800	7.99	48,788	3.34
826	−2.69	0.034	2,653,198	5.71	42,890	2.37
894	−1.93	0.015	1,912,332	5.17	38,606	5.09
895	−1.83	0.041	694,589	4.97	38,275	2.33
896	−3.09	0.015	5,361,677	5.35	38,440	2.96
1053	−2.63	0.036	2,984,453	4.99	27,203	1.65
1093	−7.66	0.015	6,128,338	7.30	24,017	2.46
1095	−4.59	0.023	1,765,854	6.92	23,914	1.91
1096	−7.55	0.015	13,937,178	7.60	23,812	0.52

DeCyder-generated list of proteins whose abundances vary by at least ±1.5-fold (p ≤ 0.05) between DOC- and water-treated OBC matrices. Columns show (left to right): numbers assigned to each spot on the gel; average ratios between spot volumes (DOC/water); p-values corresponding to those ratios; maximum spot volumes (pixels); approximate pIs and M_r (Daltons) based on position within the gel; and match quality, a measure of consistency between a spot’s replicates in different gels (lower is better). Spots above the double line are more abundant in DOC matrix, below, in water matrix
OBC osteoblastic cell

ISSN: 1757-6512