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Table 2 Identities of Proteins from "Pick List"

From: Nascent osteoblast matrix inhibits osteogenesis of human mesenchymal stem cells in vitro

 

Symbol

Entrez Gene Name

Found in Spots:

Mx

Max Score

pI

Mr

CYTOPLASM

ACTA2

actin, alpha 2, smooth muscle, aorta

826, 894–6, 1053

W

348

5.23

42,381

ALDH1B1

aldehyde dehydrogenase 1 family, member B1

658

W

283

6.36

57,658

AKR1C1

aldo-keto reductase family 1, member C1

894

W

240

8.02

37,221

ATP5A1

ATP synthase, H+ transport, mitoch F1 complex, alpha 1

658

W

867

9.16

59,828

ATP5B

ATP synthase, H+ transport, mitoch F1 complex, beta

689

W

683

5.26

56,525

CKAP4

cytoskeleton-associated protein 4

717

W

821

5.63

66,097

ENO1

enolase 1, alpha

758, 759

W

532

7.01

47,481

ENO3

enolase 3, beta, muscle

758

W

246

7.59

47,299

EIF3B

eukaryotic translation initiation factor 3, subunit B

896

W

213

5.38

36,878

FH

fumarate hydratase

758, 759

W

290

8.85

54,773

HSP90B1

heat schock protein 90kDa beta (Grp94), member 1

449, 636, 637

B

306

4.76

92,696

HSPB1

heat shock 27kDa protein 1

1053

W

315

5.98

22,826

HSPD1

heat shock 60kDa protein 1 (chaperonin)

607

W

390

5.83

60,813

HSPA5

heat shock 70kDa protein 5 (glucose-regulated)

449, 486

B

1483

5.07

72,402

IKIP

IKK interacting protein

717

W

487

9.21

39,399

PRDX1

peroxiredoxin 1

1093

W

363

8.27

22,324

P4HB

prolyl 4-hydroxylase, beta polypeptide

201, 607, 636

B

1116

4.76

57,480

PSMD13

proteasome (prosome, macropain) 26S subunit, 13

826

W

219

5.71

42,872

PDIA3

protein disulfide isomerase family A, member 3

647-9

W

706

5.98

57,146

PDIA6

protein disulfide isomerase family A, member 6

689

W

395

5.35

48,207

PDHB

pyruvate dehydrogenase (lipoamide) beta

896

W

202

6.20

39,550

PKM2

pyruvate kinase, muscle

607

W

341

7.96

58,470

SOD2

superoxide dismutase 2, mitochondrial

1093-6

W

347

8.35

24,878

TUBA1A

tubulin, alpha 1A

689, 894

W

494

4.94

50,788

ECM

COL6A1

collagen, type VI, alpha 1

201, 203, 208, 210, 224, 242, 247

D

1432

5.26

109,602

COL6A3

collagen, type VI, alpha 3

247, 607, 649

B

321

6.26

345,163

EFEMP2

EGF-containing fibulin-like ECM protein 2

636

W

297

4.79

51,725

EMILIN1

elastin microfibril interfacer 1

224-6, 242, 247

D

462

5.07

107,913

HTRA1

HtrA serine peptidase 1

637

W

267

8.09

52,167

TGFBI

transforming growth factor, beta-induced, 68kDa

486

W

247

7.62

75,261

PM

ANXA1

annexin A1

894-6

W

397

6.57

38,918

ANXA2

annexin A2

894-6

W

1201

8.44

36,950

ANXA6

annexin A6

486

W

265

5.42

76,168

FN1

fibronectin 1

all but 1093-96

D

2242

5.45

266,034

FLOT1

flotillin 1

758

W

496

7.08

47,554

NU

CRYAB

crystallin, alpha B

1093-6

W

244

6.76

20,146

LMNA

lamin A/C

561-66

W

1179

6.57

74,380

  1. Columns show (left to right): the number(s) of the spot(s) where a given protein was found; the type of matrix in which the protein was more abundant (D = DOC, W = water, B = both); maximum Mascot identity score from mass spectrometry; and theoretical pIs and Mr values determined by Mascot from the protein sequences. Bolded text indicates that the approximate pI and/or Mr of the spot in which the protein was found match its theoretical values. Proteins are grouped by subcellular location based on Ingenuity’s database
  2. NU nucleus, ECM extracellular matrix, PM plasma membrane