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Table 2 Identities of Proteins from "Pick List"

From: Nascent osteoblast matrix inhibits osteogenesis of human mesenchymal stem cells in vitro

  Symbol Entrez Gene Name Found in Spots: Mx Max Score pI Mr
CYTOPLASM ACTA2 actin, alpha 2, smooth muscle, aorta 826, 894–6, 1053 W 348 5.23 42,381
ALDH1B1 aldehyde dehydrogenase 1 family, member B1 658 W 283 6.36 57,658
AKR1C1 aldo-keto reductase family 1, member C1 894 W 240 8.02 37,221
ATP5A1 ATP synthase, H+ transport, mitoch F1 complex, alpha 1 658 W 867 9.16 59,828
ATP5B ATP synthase, H+ transport, mitoch F1 complex, beta 689 W 683 5.26 56,525
CKAP4 cytoskeleton-associated protein 4 717 W 821 5.63 66,097
ENO1 enolase 1, alpha 758, 759 W 532 7.01 47,481
ENO3 enolase 3, beta, muscle 758 W 246 7.59 47,299
EIF3B eukaryotic translation initiation factor 3, subunit B 896 W 213 5.38 36,878
FH fumarate hydratase 758, 759 W 290 8.85 54,773
HSP90B1 heat schock protein 90kDa beta (Grp94), member 1 449, 636, 637 B 306 4.76 92,696
HSPB1 heat shock 27kDa protein 1 1053 W 315 5.98 22,826
HSPD1 heat shock 60kDa protein 1 (chaperonin) 607 W 390 5.83 60,813
HSPA5 heat shock 70kDa protein 5 (glucose-regulated) 449, 486 B 1483 5.07 72,402
IKIP IKK interacting protein 717 W 487 9.21 39,399
PRDX1 peroxiredoxin 1 1093 W 363 8.27 22,324
P4HB prolyl 4-hydroxylase, beta polypeptide 201, 607, 636 B 1116 4.76 57,480
PSMD13 proteasome (prosome, macropain) 26S subunit, 13 826 W 219 5.71 42,872
PDIA3 protein disulfide isomerase family A, member 3 647-9 W 706 5.98 57,146
PDIA6 protein disulfide isomerase family A, member 6 689 W 395 5.35 48,207
PDHB pyruvate dehydrogenase (lipoamide) beta 896 W 202 6.20 39,550
PKM2 pyruvate kinase, muscle 607 W 341 7.96 58,470
SOD2 superoxide dismutase 2, mitochondrial 1093-6 W 347 8.35 24,878
TUBA1A tubulin, alpha 1A 689, 894 W 494 4.94 50,788
ECM COL6A1 collagen, type VI, alpha 1 201, 203, 208, 210, 224, 242, 247 D 1432 5.26 109,602
COL6A3 collagen, type VI, alpha 3 247, 607, 649 B 321 6.26 345,163
EFEMP2 EGF-containing fibulin-like ECM protein 2 636 W 297 4.79 51,725
EMILIN1 elastin microfibril interfacer 1 224-6, 242, 247 D 462 5.07 107,913
HTRA1 HtrA serine peptidase 1 637 W 267 8.09 52,167
TGFBI transforming growth factor, beta-induced, 68kDa 486 W 247 7.62 75,261
PM ANXA1 annexin A1 894-6 W 397 6.57 38,918
ANXA2 annexin A2 894-6 W 1201 8.44 36,950
ANXA6 annexin A6 486 W 265 5.42 76,168
FN1 fibronectin 1 all but 1093-96 D 2242 5.45 266,034
FLOT1 flotillin 1 758 W 496 7.08 47,554
NU CRYAB crystallin, alpha B 1093-6 W 244 6.76 20,146
LMNA lamin A/C 561-66 W 1179 6.57 74,380
  1. Columns show (left to right): the number(s) of the spot(s) where a given protein was found; the type of matrix in which the protein was more abundant (D = DOC, W = water, B = both); maximum Mascot identity score from mass spectrometry; and theoretical pIs and Mr values determined by Mascot from the protein sequences. Bolded text indicates that the approximate pI and/or Mr of the spot in which the protein was found match its theoretical values. Proteins are grouped by subcellular location based on Ingenuity’s database
  2. NU nucleus, ECM extracellular matrix, PM plasma membrane