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Fig. 12 | Stem Cell Research & Therapy

Fig. 12

From: Genetic modification of human mesenchymal stem cells helps to reduce adiposity and improve glucose tolerance in an obese diabetic mouse model

Fig. 12

Intracellular SOD assay: detection of SOD activity in SOD1 and SOD2 upregulated cells compared to AdNull transduced cells. SOD superoxide dismutase. To ensure that superoxide dismutase (SOD) has been truly upregulated intracellularly we assessed SOD activity (Fig. 12) and demonstrated that SOD2 upregulated MSCs have more dismutase activity and used less xanthine oxidase compared to SOD1 upregulated cells indicating that SOD2 upregulated cells have more SOD activity than SOD1. Xanthine oxidase (XO) is a key enzyme necessary to produce superoxide. AdNull transduced cells were associated with maximum XO, as expected, and thereby was associated with least SOD activity

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Stem Cell Research & Therapy

ISSN: 1757-6512