Fig. 2
From: Transplantation of hESC-derived hepatocytes protects mice from liver injury
Differentiation of hepatoblasts into precursors of cholangiocytes. a Protocol and phase contrast images of cholangiocyte precursors. Hepatic progenitors were passaged at day 11 onto collagen 1-coated wells and grown for 2 days in HamF12/Williams (HPM), 20 ng/ml HGF, then for 2 days in HPM, 20 ng/ml HGF and 20 ng/ml epidermal growth factor (EGF). From day 16 to day 18 of differentiation cells were grown in a mixture of HPM/HCM. b Representative field of immunostaining of cholangiocyte precursor cells. Cells express osteopontin (OPN), hepatic nuclear factor (HNF1) 1β , cytokeratin (CK)7, CK18, cystic fibrosis transmembrane receptor (CFTR) and are negative for HNF4α, a hepatocyte transcription factor. c Representative phase contrast image (×40) of cysts obtained 1 week after seeding in matrigel at day 18 of differentiation (left panel). Immunostaining of cyst (middle panel) and tubule (right panel) showing the polarity of the cells in these structures. GH Growth hormone, IL Interleukin, TGF Transforming growth factor