Fig. 1
From: Bioprocessing strategies for the large-scale production of human mesenchymal stem cells: a review
Expansion of bone marrow-derived human mesenchymal stem cells (BM-hMSCs) on Cytodex 3 microcarriers in serum-free PPRF-msc6 medium in 125-ml stirred suspension bioreactors [84]. hMSCs isolated in PPRF-msc6 were thawed and expanded for two passages in PPRF-msc6 and then inoculated at 2.4 × 104 cells/ml in stirred suspension bioreactors containing 2.0 g/l of Cytodex 3 microcarriers. We observed variable cell growth kinetics between different BM donors (BM1, BM2, and BM3). This indicates that although this bioreactor system was optimized for the growth of one hMSC donor, inter-donor differences influence the growth kinetics of hMSCs in stirred suspension bioreactors. Error bars represent the observed range, n = 2