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Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: Cryopreservation of equine mesenchymal stem cells in 95 % autologous serum and 5 % DMSO does not alter post-thaw growth or morphology in vitro compared to fetal bovine serum or allogeneic serum at 20 or 95 % and DMSO at 10 or 5 %

Fig. 1

Total viable cell number. Viability of MSCs from nine horses cryopreserved in six different freezing solutions after a 24 hours and b 72 hours in monolayer culture post thaw (median, quartiles). There were no differences between the groups (24 hours, p = 0.96; 72 hours, p = 0.51). a Dotted line represents the total number of viable MSCs seeded post thaw. The total viable MSCs at 24 hours post thaw were significantly lower than the cell number seeded in all groups (20/10/70FBS, p = 0.02; 20/10/70Allo, p = 0.05; 20/10/70Auto, p = 0.01; 95/5FBS, p = 0.05; 95/5Allo, p = 0.05; 95/5Auto, p = 0.02). Allo allogenic, Auto autologous, FBS fetal bovine serum, 20/10/70 20 % serum, 10 % dimethyl sulfoxide, 70 % minimum essential media, 95/5 95 % serum, 5 % dimethyl sulfoxide

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