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Table 1 Age of mesenchymal stem cell donors and passage number

From: Cryopreservation of equine mesenchymal stem cells in 95 % autologous serum and 5 % DMSO does not alter post-thaw growth or morphology in vitro compared to fetal bovine serum or allogeneic serum at 20 or 95 % and DMSO at 10 or 5 %

 

Morphology

Debris

Excellent/none

Cells were spindle shaped

<5 floating cells per 40× field

Good/mild

Cells were wider or more star shaped

<20 floating cells per 40× field

Fair/moderate

Cells were flattened and/or contained large vacuoles

<40 floating cells per 40× field

Poor/severe

Cells were flattened, vacuolar, and foamy in appearance

≥40 floating cells per 40× field

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Stem Cell Research & Therapy

ISSN: 1757-6512