Fig. 1

Phenotypic and functional validation of BM-MSCs and AT-MSCs. a BM-MSCs and AT-MSCs analysed for cell surface antigen expression of known positive (CD29, CD44, CD73, and CD90) and negative (CD34 and CD45) MSC markers. Red closed histograms represent MSCs labelled with antibodies against the surface antigen indicated on the right hand side of each row. Blue open histograms show isotype controls. BM-MSCs b and AT-MSCs b′ adhered to plastic with a perceptible appearance typical of MSCs in culture. Scale bar = 200 μm. BM-MSCs and AT-MSCs cultured without c-d and with c′-d′ adipogenesis differentiation medium for 14 days and stained with Oil red O. Scale bar = 50 μm. BM-MSCs and AT-MSCs cultured without e-f and with e′-f′ osteogenesis differentiation medium for 21 days and stained with Alizarin red S. Scale bar = 200 μm. BM-MSCs bone marrow-derived mesenchymal stem cells, AT-BMCs adipose tissue-derived mesenchymal stem cells