Fig. 4
Effects of MSCs on leukocyte infiltration to the myenteric plexus. a-d′ CD45-IR leukocytes (green) visualised on the level of myenteric neurons labelled with anti-PGP9.5 (red) by confocal microscopy in LMMP wholemounts prepared from colon collected at 24 a-d and 72 h a′-d′ post treatment. Scale bar = 50 μm. e CD45-IR leukocytes quantified in a 2 mm2 area of the myenteric plexus in the colon. *p < 0.05, **p < 0.01, ***p < 0.001, n = 4 animals/group/time point. MSCs mesenchymal stem cells, LMMP longitudinal muscle and myenteric plexus