Fig. 4

Interaction of peptides with human MSCs in suspension. Human bmMSCs (a) and pMSCs (b) were expanded to the second passage of in vitro culture, harvested by mild proteolysis, and stained with titrated amounts of FITC-labeled peptides. In addition, bmMSCs were counterstained with PE-labeled anti-CD90 (a), and pMSCs with PE-labeled anti-CD73 (b) to confirm their mesenchymal origin. Moderate binding of peptides P7, P13, and P15 was observed on bmMSCs (a). In contrast, pMSCs displayed a bright staining with all peptides investigated (b). FITC Fluorescein isothiocyanate, FSC Forward scatter, PE Phycoerythrin, SSC Side scatter