Fig. 5

Interaction of peptides with human bmMSCs ex vivo. Mononuclear cells were purified from human bone marrow by Ficoll® gradient centrifugation, washed and incubated with peptides as indicated and counterstained with AF-647-labeled mAb to CD271, a marker for bmMSCs. Peptide P13 stained a small subset of CD271^pos bone marrow cells ex vivo, whereas peptides P4, P14, and P15 stained only very few CD271^pos cells. Peptides P7 failed to bind to CD271^pos cells, but all peptides interacted with CD271^neg bone marrow cells. FITC Fluorescein isothiocyanate, FSC Forward scatter, SSC Side scatter