Skip to main content
Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: Adenovirus-mediated transfer of hepatocyte growth factor gene to human dental pulp stem cells under good manufacturing practice improves their potential for periodontal regeneration in swine

Fig. 1

Characterization of hDPSCs cultured under GMP conditions. a Microscopic image (left) and fluorescence microscopic image (right) of MSCs transfected with Ad-GFP at a MOI of 150 for 48 hours. b Transfection efficiency of MSCs transfected with Ad-GFP. Cells were transfected at different MOI levels and cultured for 48 hours. Transfection efficiency was determined by flow cytometry. c Adenovirus-mediated expression of HGF in hDPSCs infected with Ad-HGF at an MOI of 150. Conditioned medium was collected at different time points, and HGF content was determined by ELISA. *P <0.05 for hDPSCs infected with Ad-HGF compared with Ad-GFP control. d Flow cytometric analysis of hDPSCs and HGF-hDPSCs shows expression of cell markers CD73, CD105, CD90, and CD146, and negative results for HLA-DR and CD45. d days, GFP green fluorescence protein, hDPSC human dental pulp stem cell, HGF hepatocyte growth factor, PE phycoerythrin

Back to article page