Fig. 1From: Adenovirus-mediated transfer of hepatocyte growth factor gene to human dental pulp stem cells under good manufacturing practice improves their potential for periodontal regeneration in swineCharacterization of hDPSCs cultured under GMP conditions. a Microscopic image (left) and fluorescence microscopic image (right) of MSCs transfected with Ad-GFP at a MOI of 150 for 48Â hours. b Transfection efficiency of MSCs transfected with Ad-GFP. Cells were transfected at different MOI levels and cultured for 48Â hours. Transfection efficiency was determined by flow cytometry. c Adenovirus-mediated expression of HGF in hDPSCs infected with Ad-HGF at an MOI of 150. Conditioned medium was collected at different time points, and HGF content was determined by ELISA. *P <0.05 for hDPSCs infected with Ad-HGF compared with Ad-GFP control. d Flow cytometric analysis of hDPSCs and HGF-hDPSCs shows expression of cell markers CD73, CD105, CD90, and CD146, and negative results for HLA-DR and CD45. d days, GFP green fluorescence protein, hDPSC human dental pulp stem cell, HGF hepatocyte growth factor, PE phycoerythrinBack to article page