Skip to main content
Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: Autophagy promotes apoptosis of mesenchymal stem cells under inflammatory microenvironment

Fig. 3

Inhibition of autophagy promotes survival of MSCs via upregulation of Bcl-2. a shNC-MSCs (shNC) and shBec1-MSCs (shBecn1) were stimulated with tumor necrosis factor alpha (TNF-α; 20 ng/ml) plus interferon gamma (IFN-γ; 50 ng/ml) for 4 hours. Expression of Bcl-2, Bcl-xl, Mcl-1 and Bim was then measured by quantitative real-time PCR. Data are shown as mean ± SEM of four independent experiments. b Western blot analysis of shNC-MSCs and shBec1-MSCs stimulated with TNF-α (20 ng/ml) plus IFN-γ (50 ng/ml) for 6 hours. c, d shNC-MSCs or shBec1-MSCs were mixed with matrigel and were injected into naive (n = 3 mice per group) and cecal ligation and puncture (CLP) mice (n = 3 mice per group) subcutaneously for 24 hours. Expression of Bcl-2 was then measured by quantitative real-time PCR (c) and western blot (d). e shNC-MSCs and shBec1-MSCs were stimulated with TNF-α (20 ng/ml) plus IFN-γ (50 ng/ml), or with PD98059 (20 μM) or N-acetyl cysteine (NAC; 10 mM) for 4 hours. Expression of Bcl-2 was then measured by quantitative real-time PCR. Data are shown as mean ± SEM of four independent experiments. f MSCs were pretreated with 3-methyladenine (3-Ma) for 12 hours, and stimulated with TNF-α (20 ng/ml) and IFN-γ (50 ng/ml), or combined with PD98059 (20 μM) or NAC (10 mM) for 4 hours. The expression of Bcl-2 was then measured by quantitative real-time PCR. Data are shown as mean ± SEM of four independent experiments. *P < 0.05, **P < 0.01

Back to article page