Fig. 1
ALP expression and molecular analysis of epiphyseal and diaphyseal populations. Isolated cell populations (P1) were seeded into CFU-F assay (1 × 101 cells/cm2) and cultured for 14 days prior to quantification of ALP+ colonies a and b. Populations were cultured in T25 cm2 flasks, and colonies were counted from the whole flask. ALP+ colonies are presented as a percentage of the total number of colonies. Molecular analysis in epiphyseal and diaphyseal populations assessed expression of osteochondral genes after 14 days culture in basal medium, including OSX c, RUNX2 d, ALP e, COL1A1 f, ON g, OCN h, COL10A1 i, SOX9 j, and COL2A1 k. Error bars are SD. * P ≤ 0.05, ** P ≤ 0.01, *** P ≤ 0.001 (foetal samples, n = 3; 55 61 and 63 days post conception). ALP alkaline phosphatase, CFU-F colony forming unit – fibroblastic