Fig. 4
In vitro characterisation of epiphyseal and diaphyseal 3D pellet cultures. 2.5 × 105 cells per pellet (P2) were cultured in basal medium for 48 h before transfer to differentiation medium for 21 days in organotypic culture. Diaphyseal pellets were cultured in basal and osteogenic medium, whilst epiphyseal pellets were cultured in basal and chondrogenic medium. Specific ALP activity was also assessed following 21 days culture. Scale bars measure 200 μm. (foetal samples, n = 6; 55, 55, 56, 63, 63 and 65 days post conception). ALP alkaline phosphatase