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Fig. 5 | Stem Cell Research & Therapy

Fig. 5

From: Regionally-derived cell populations and skeletal stem cells from human foetal femora exhibit specific osteochondral and multi-lineage differentiation capacity in vitro and ex vivo

Fig. 5

Ex vivo bone defect regeneration following implantation of diaphysis-derived a and epiphysis-derived b 3D cell pellets. Pellets (1.5 × 104 cells per pellet [P2]) were implanted in drill defects (300 μm diameter) at both epiphyseal and diaphyseal locations on an embryonic day 11 chick femur, and incubated for 10 days in organotypic culture in basal medium. Femora were fixed, embedded in wax, sectioned and stained with Alcian blue/Sirius red and imaged for birefringence using polarising filters. Highly aligned collagen fibres in mineralised bone appeared red. Scale bars measure 1 mm (low magnification images) and 100 μm (high magnification images). (foetal samples, n = 4; 54, 59, 63 and 65 days post conception)

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