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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: Tissues from equine cadaver ligaments up to 72 hours of post-mortem: a promising reservoir of stem cells

Fig. 2

Tri-lineage differentiation potential of EC-MSC at P3. Extracellular calcium deposits stained with Alizarin Red S showed the differentiation of EC-MSC into the osteoblast lineage, whereas no calcium deposits were observed in non-induced cells (a). The accumulation of intracytoplasmic lipid droplets stained with Oil Red O indicated the adipogenic differentiation of EC-MSC, whereas no lipid droplets were observed in non-induced cells (b). Positive Alcian Blue staining of proteoglycan production confirmed the chondrogenic differentiation of EC-MSC, as compared to the absence of staining of control cells (c). Bars, 200 μm. EC-MSC equine cadaver mesenchymal stem cell

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