Fig. 4

MATLAB analysis of vessel network complexity. MATLAB analysis of confocal images (scale bars = 250 μm) (i) generated new images (ii) showing vascular network elements (grey), upon which differently colored complexity factor values are presented. These values were divided into three broad ranges: a red <0.3, yellow 0.3–0.55 and blue >0.55. b Blue elements represent rounded single cells and yellow elements the more elongated structures. c Blue and yellow elements represent rounded cells and elongated structures, respectively, combined with more red elements, representing long and complex vessels. d  Dominance of red elements representing long and complex vessels and less yellow and blue elements for rounded shapes