Fig. 6

Vessel alignment as an indication of vessel maturity. a Representative image of half of a scaffold showing different regions with orientated vessels (scale bar = 500 μm). b Magnified image of vessels orientated in a specific direction (scale bar = 100 μm) and c a histogram showing the distribution of the elements orientation in image b (represented by different angles). d A magnified image of non-oriented vessels (scale bar = 100 μm) and e a histogram showing the distribution of the elements orientation in image d (represented by different angles). f Statistical analysis of the number of elements in a given direction versus the total number of elements (%) in each field of view. Two-way ANOVA, Bonferroni’s multiple comparisons test, n ≥ 4. ****p < 0.0001. HAMEC Human adipose microvascular endothelial cell, HNDF Human neonatal dermal fibroblast, HUVEC Human umbilical vein endothelial cell, MSC Mesenchymal stem cell