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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: Alternative source of stem cells derived from human periodontal ligament: a new treatment for experimental autoimmune encephalomyelitis

Fig. 2

Clinical score, body weight, H&E and LFB. Mice were immunized with MOG35–55 and monitored every 2 days for clinical disease score of experimental autoimmune encephalomyelitis (EAE) and body weight gain/loss for 56 days. a Normal, naive mice did not display motor deficit. EAE mice displayed a grading of disease with a mean clinical score of 3.05, while mice treated with human periodontal ligament stem cells (hPDLSCs) revealed a lower grade of disability with a mean clinical score of 1.05. The measure of clinical disease score is expressed as mean ± SEM of all measurements of each experimental group. ****p < 0.0001. b The variation of body weight has been expressed compared to day of EAE induction (day 0) as mean ± SEM of all animals for each experimental group. Naive mice showed a normal increase in body weight. On the contrary, a significant body weight loss was observed in EAE mice, whereas a relevant body weight gain was found in hPDLSC-treated mice. ****p < 0.0001. hPDLSC treatment improved inflammatory cell infiltration caused by EAE. The severity of the damage was evaluated 56 days after EAE induction by H&E staining. c Naive mice did not show histological alterations in the spinal cord tissues. d H&E staining for EAE mice displayed a wide area of infiltrating cells (arrows) confirming the important role of the cellular components that undergoes degenerative conditions. e Conversely, treatment with hPDLSCs led to a complete resolution of inflammatory cells infiltration. g In addition, EAE untreated animals exhibited remarkably reduced myelin in the spinal cord (area in brackets), whereas naive mice did not show demyelination plaques (c and f). h Moreover, treatment with hPDLSC reduces demyelination and axonal loss in EAE mice with an intense LFB positive staining (see area in brackets)

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