Fig. 4

Immunohistochemical evaluation for CD4, CD8α, IL-1β and IL-10. CD4 and CD8α expression reveals higher tissue levels of these markers in experimental autoimmune encephalomyelitis (EAE)-affected mice (b, 20×, B1 magnification, 40×; e, 20×, E1 magnification, 40×) compared to the human periodontal ligament stem cell (hPDLSC)-treated group (c, f). Spinal cord specimens from naive mice did not stain for CD4 as well as for CD8 (a, d). Increased IL-1β tissue localization in EAE mice was reported (e, h). On the contrary, reduced expression of IL-1β was observed in mice that received hPDLSCs (i). g The immunohistochemical analysis showed that no positive staining for IL-1β was observed in the tissues obtained from naive mice. k EAE sections did not stain for IL-10 antibody. l Positive IL-10 expression levels were found in the EAE group treated with hPDLSCs (arrows; l, 20×, L1 magnification, 40×). j Naive mice showed negative staining for IL-10