Fig. 1
From: Allogeneic guinea pig mesenchymal stem cells ameliorate neurological changes in experimental colitis
Immunophenotype and clonogenicity of guinea pig MSCs from bone marrow and adipose tissue. a GpBM-MSC and gpAT-MSCs were analysed for cell surface antigen expression of known positive MSC markers CD29 and CD73. Red closed histograms represent MSCs labelled with antibodies against the surface antigen indicated on the right-hand side of each row. Blue open histograms show isotype controls. GpBM-MSCs (b) and gpAT-MSCs (b′) adhered to plastic with a perceptible appearance typical of MSCs in culture. Scale bar = 200 μm. The clonogenicity of gpBM-MSCs (c) and gpAT-MSCs (c′) was determined by a colony-forming unit-fibroblast (CFU-f) assay (n = 4 independent cultures per group). d CFU-f counts were quantified as a percentage of the total viable cells seeded. ****P <0.0001. gpAT-MSC guinea pig adipose tissue-derived mesenchymal stem cell, gpBM-MSC guinea pig bone marrow-derived mesenchymal stem cell, MSC mesenchymal stem cell