Allogeneic guinea pig mesenchymal stem cells ameliorate neurological changes in experimental colitis

Table 3 Effects of allogeneic mesenchymal stem cells derived from guinea pigs on myenteric neurons in TNBS-induced colitis

	Sham	TNBS	TNBS + gpBM-MSC	TNBS + gpAT-MSC
	Total number of myenteric neurons/2 mm²
24 h	262.6 ± 2.9	204.6 ± 6.9††††	238.8 ± 6.2*	241.3 ± 8.6**
72 h	261.8 ± 3.9	203.4 ± 4.2†††	241.0 ± 12.7*	238.0 ± 13.2*
	Total number of nNOS-IR neurons/2 mm²
24 h	51.8 ± 1.5	68.8 ± 3.9††	58.5 ± 4.3*	55.5 ± 4.4**
72 h	53.0 ± 1.0	67.6 ± 3.8†	56.0 ± 5.5*	55.8 ± 1.8**
	Proportion of nNOS-IR neurons/2 mm², %
24 h	19.8 ± 0.8	33.8 ± 2.4†††	24.6 ± 2.2**	23.0 ± 1.7***
72 h	20.3 ± 0.3	33.3 ± 1.9†††	23.3 ± 2.0**	23.6 ± 1.3**
	Total number of ChAT-IR neurons/2 mm²
24 h	159.6 ± 3.9	111.4 ± 4.0††††	134.3 ± 5.7†**	135.3 ± 5.7†**
72 h	157.4 ± 4.5	110.2 ± 2.2††††	137.8 ± 1.5†**	135.3 ± 2.5†**
	Proportion of ChAT-IR neurons/2 mm², %
24 h	60.8 ± 1.6	54.6 ± 2.3	56.7 ± 4.1	56.0 ± 1.3
72 h	60.1 ± 1.2	54.3 ± 2.0	57.6 ± 3.0	57.2 ± 2.1

TNBS 2,4,6-trinitrobenzene sulfonic acid, gpBM-MSC guinea pig bone marrow-derived mesenchymal stem cell, gpAT-MSC guinea pig adipose tissue-derived mesenchymal stem cell, nNOS neuronal nitric oxide synthase, ChAT choline acetyltransferase, IR immunoreactive. †P <0.05, ††P <0.01, †††P <0.001, ††††P <0.0001, significantly different from sham; *P <0.05, **P <0.01, ***P <0.001, significantly different from TNBS

ISSN: 1757-6512