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Fig. 5 | Stem Cell Research & Therapy

Fig. 5

From: Combining mesenchymal stem cell sheets with platelet-rich plasma gel/calcium phosphate particles: a novel strategy to promote bone regeneration

Fig. 5

a Dose effects of CaP particles on BMSC proliferation after culturing for 1, 3 and 7 days (mean ± SD) (*P <0.05). b Gene expressions of BMSC sheet cultured with CaP particles or/and PRP for 7 days (*P <0.05). c Gene expressions of BMSCs cultured with CaP particles or/and PRP for 7 days (*P <0.05). d Osteocalcin, collagen I and vascular epithelial growth factor (VEGF) gene expression of the BMSC sheet and cultured BMSCs (*P <0.05). e, f, g The alizarin red staining of BMSCs cultured with PRP/CaP particles, CaP particles or without treatment for 14 days. e The alizarin red staining in the controls was much weaker. f The alizarin red staining was increased when treated with CaP particles. g A calcification node formed in the BMSCs co-cultured with CaP particles/PRP. h ALP activity of BMSCs cultured with PRP/CaP particles, CaP particles or without treatment for 14 days; ALP activity was 3.01 ± 0.51 nmol/s/mg protein, 1.95 ± 0.36 nmol/s/mg protein and 0.79 ± 0.08 nmol/s/mg protein in the CaP particles/PRP group, CaP particles group and control group respectively. i: Quantification of alizarin red staining of BMSCs cultured with PRP/CaP particles, calcium phosphate particles or without treatment for 14 days; expression of BMSCs co-cultured with CaP particles/PRP was 0.84 ± 0.07, the cells with CaP particles was 0.4 ± 0.06 and the control was 0.09 ± 0.01. j, k, l Calcein blue staining of BMSCs without treatment (j) or cultured with CaP particles (k) or PRP/CaP particles (l) for 14 days

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