Fig. 6

Fluorescence images obtained by a small animal in vivo fluorescence imaging system at 1 day (a), 3 days (b), 7 days (c), 2 weeks (d), 4 weeks (e) after the bone defects were treated with CM-Dil-labelled BMSC sheet/PRP gel/CaP particles. Red fluorescence in the bone defect zones was visualised using a small animal in vivo fluorescence imaging system, which confirmed the presence of the implanted BMSCs. After further frozen sectioning, red fluorescence was also visible in the defect zones by confocal microscopy at 1 day (f), 3 days (g), 7 days (h), 2 weeks (i) and 4 weeks (j). However, the fluorescence intensity gradually weakened. The red and the blue colours were stained by CM-Dil and Hoechst 33258, respectively