Cell and mitochondria morphology. Cell and mitochondria morphology was evaluated by chemifluorescence 2 days after thawing (n=8). The cells were stained with Alexa Fluor 488-phalloidin (green) to visualize actin filaments and MitoTracker Red (LifeTech, M7512) to evaluate mitochondria membrane stability (a). We used 4′,6-diamidino-2-phenylindole to counterstain the cell nuclei (blue). Scale bars represent 20 μm. CMXRos fluorescence intensity was measured with ImageJ and the average and standard error of the mean (SEM) were plotted as relative to the value of FBS control group (b). Numbers of small round cells were counted with ImageJ software and mean number of cells per mm2 of each group is presented with SEM (c).