Fig. 5
Post-cryopreservation senescence marker activity. Cryopreserved human amniotic epithelial cells were cultured for 21 days and the influence of cryopreservation was evaluated by senescence-associated-beta-galactosidase (SA-βgal) activity, which is detectable in the cytoplasmic blue color staining (a). The ratio of SA-β-Gal-positive cells was calculated and demonstrated as mean ± standard error of the mean (b)