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Fig. 7 | Stem Cell Research & Therapy

Fig. 7

From: Efficient differentiation of human embryonic stem cells to arterial and venous endothelial cells under feeder- and serum-free conditions

Fig. 7

In vivo safety and functionality assessment of hESC-derived arterial and venous endothelial cells. a Representative hematoxylin and eosin (H&E) stained micrographs of Matrigel plug study showing absence of microvessels in Matrigel-only control, while the others show the formation of perfused microvessels by engrafted hESC-derived arterial and venous ECs. b Histochemical micrographs of microvessels formed by hESC-derived arterial and venous ECs stained for hCD31, hCollagen-IV, hEphrin-B2 and hEph-B4. c Representative photographs showing the presence or absence of teratoma formation in immunodeficient mice. d Representative H&E stained micrographs showing the formation of teratoma by H1-hESCs with evidence of differentiation to derivatives of three germ layers: ectoderm (hair follicles, <), mesoderm (cartilage, *), and endoderm (glandular structures, +). e Representative H&E stained micrographs showing the absence of teratoma formation by H1-hESCs derived arterial and venous ECs. Scale bars in (a) and (b) = 100 μm; in (d) and (e) = 500 μm. Art-EC Arterial endothelial cells, hESC Human embryonic stem cells, Ven-EC Venous endothelial cells

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