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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: Chondrogenic induction of mesenchymal stromal/stem cells from Wharton’s jelly embedded in alginate hydrogel and without added growth factor: an alternative stem cell source for cartilage tissue engineering

Fig. 2

Changes in MSC viability during scaffold culture. Cell viability was measured by flow cytometry at 3, 14 and 28 days of culture of MSC embedded in Alg/HA hydrogel. Necrotic and apoptotic cells were labeled with propidium iodide and annexin V–Alexa 488, respectively. a Positive controls for apoptotic and necrotic cells. b Cell viability was evaluated after spraying method of scaffold construct between BM-MSC and WJ-MSC. c WJ-MSC viability was evaluated for two methods of scaffold construct: alginate beads and alginate cylinders (obtained by spraying method) at 3, 7 and 10 days of culture. The results are expressed as mean ± standard error of the mean (n ≥ 3). *p < 0.05 and **p < 0.01, day x vs day 3 for the same cell source (b) or method of scaffold construct (c). # p < 0.05, cylinders vs beads for the same culture time. BM-MSC bone marrow-derived mesenchymal stromal/stem cells, WJ-MSC Wharton’s ielly-derived mesenchymal stromal/stem cells

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Stem Cell Research & Therapy

ISSN: 1757-6512