Fig. 2

Mutation of OPA1 increases necrosis and apoptosis in iPSCs. a, b Control and VO-iPSCs were stained with propidium iodide and YO-PRO-1 dye using an Apoptotic kit (Invitrogen) to detect necrotic cells (red, left panels) and apoptotic cells (green, right panels), respectively. Scale bars = 100 μM. c Each column represents the number (mean counts) of either necrotic or apoptotic cells from control and VO-iPSCs. The number of necrotic and apoptotic VO-iPSCs was significantly higher compared to that of the control iPSCs. The values shown are given as mean ± SD (*p < 0.05, **p < 0.001). SD is shown inside the boxes. iPS induced pluripotent stem