Fig. 3

OPA1 ^+/−-iPSCs are unable to differentiate into neural rosettes (NR). Embryoid bodies (EBs) were cultured with hESC medium (without bFGF) on ultra-low attachment plates. Day 5 Ebs derived from the control iPSCs were shown in (a) (left panel). EBs were well organized and exhibited a round shape. In contrast, EBs derived from VO-iPSCs (a, right panel) exhibited irregular edges and did not have a round shape. EBs were subsequently plated onto PLO/L-coated wells and were cultured with hESC medium containing 10 % FBS. b Cells were imaged 5 days after EB attachment. c IF staining with a ZO-1 antibody (green) was used to show neural rosette structures. Scale bars = 20 μM